Detection of herpes simplex virus type-specific antibodies by an enzyme-linked immunosorbent assay based on glycoprotein G

1997 ◽  
Vol 53 (4) ◽  
pp. 319-323 ◽  
Author(s):  
Madoka Hashido ◽  
Francis K. Lee ◽  
Sakae Inouye ◽  
Takashi Kawana
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Immunosorbent Assay ◽  
Herpes Simplex Virus Type ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Antibodies ◽  
Glycoprotein G ◽  
Simplex Virus

scholarly journals Premarket Evaluation of a Commercial Glycoprotein G-Based Enzyme Immunoassay for Herpes Simplex Virus Type-Specific Antibodies

1998 ◽  
Vol 36 (1) ◽  
pp. 294-295 ◽  
Author(s):  
Rhoda L. Ashley ◽  
Linxian Wu ◽  
Jerry W. Pickering ◽  
Ming-Chieh Tu ◽  
Laura Schnorenberg
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Enzyme Immunoassay ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Glycoprotein G ◽  
Simplex Virus ◽  
Hsv 1

A new commercial glycoprotein G-based enzyme immunoassay (gG-EIA) was compared with Western blotting (WB) for detection of herpes simplex virus type 1 (HSV-1) or HSV-2 type-specific antibodies in 193 serum samples. Sensitivity for HSV-1 was 95%; specificity was 96%. Sensitivity for HSV-2 was 98%; specificity was 97%. Twelve of 13 serum samples with equivocal gG-EIA results were serotyped by WB.

scholarly journals Performance of Two Commercial Enzyme-Linked Immunosorbent Assay Kits Using Recombinant Glycoprotein G2 Antigen for Detection of Herpes Simplex Virus Type 2 Specific Antibodies

2005 ◽  
Vol 12 (2) ◽  
pp. 359-360 ◽  
Author(s):  
Sharmila M. Reddy ◽  
P. Balakrishnan ◽  
S. Uma ◽  
S. P. Thyagarajan ◽  
Suniti Solomon
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Immunosorbent Assay ◽  
Herpes Simplex Virus Type ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Western Blot Assay ◽  
Simplex Virus

ABSTRACT For 93 stored serum samples tested by HerpeSelect2 and the Euroimmun enzyme-linked immunosorbent assay for detection of herpes simplex virus type 2-specific immunoglobulin G antibodies, the concordance of positive and negative results was 100%. Moreover, all the results that were equivocal by HerpeSelect2 (negative by Euroimmun) were confirmed as being negative by a Western blot assay.

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